2017 Publications

Association between gaseous air pollution and hospital admissions for hypertension in Taipei, Taiwan
Chen, CC;Yang, CY
Abstract:Air pollution exposure has been reported to influence blood pressure. However, only a few epidemiological studies demonstrated association between ambient air pollution exposure and acute hypertensive events. The aim of this study was to examine the association between gaseous air pollutants exposure and hospital admissions for hypertension in Taipei, Taiwan. Data on hospital admissions for hypertension and ambient air pollution in Taipei were obtained for the 2009-2013 period. (more)


Occupational hazard exposures and depressive symptoms of pregnant workers
Yeh, SS;Lee, CN;Wu, YH;Tu, NC;Guo, YL;Chen, PC;Chen, CH
Abstract:OBJECTIVES: To explore the prevalence of exposure to occupational hazards and depressive mood with associated underlying risk factors among pregnant workers. METHODS: Women at 12 weeks of gestation (n = 172) were recruited during regular prenatal screening. Data were obtained via questionnaires that explored job details and Edinburgh Postnatal Depression Scale.(more)


Development of an in vitro-based risk assessment framework for predicting ambient particulate matter-bound polycyclic aromatic hydrocarbon-activated toxicity pathways
Chou, WC;Hsu, CY;Ho, CC;Hsieh, JH;Chiang, HC;Tsou, TC;Chen, YC;Lin, P
Abstract:Polycyclic aromatic hydrocarbons (PAHs) are widely distributed throughout the atmosphere as mixtures attached to ambient particulate matter (PM). PAHs usually elicit similar toxicological pathways but do so with varying levels of efficacy. In this study, we utilized high-throughput screening (HTS) in vitro data of PAHs to predict health risks associated with coarse and fine PM. PM samples with 22 PAH compounds obtained from residential areas close to industrial parks in central Taiwan were analyzed. (more)


Comparative microarray analyses of mono(2-ethylhexyl)phthalate impacts on fat cell bioenergetics and adipokine network
Chiang, HC;Wang, CH;Yeh, SC;Lin, YH;Kuo, YT;Liao, CW;Tsai, FY;Lin, WY;Chuang, WH;Tsou, TC
Abstract:Cellular accumulation of mono(2-ethylhexyl)phthalate (MEHP) has been recently demonstrated to disturb fat cell energy metabolism; however, the underlying mechanism remained unclear. The study aimed to determine how MEHP influenced fat cell transcriptome and how the changes might contribute to bioenergetics. (more)


Estrogenic chemicals at body burden levels attenuate energy metabolism in 3T3-L1 adipocytes
Tsou, TC;Yeh, SC;Hsu, JW;Tsai, FY
Abstract:The study aimed to examine effects of environmental estrogens at body burden levels on energy metabolism in fat cells. Acclimation of T47D-KBluc cells in estrogen-deprived medium was established for high performance of estrogen-responsive luciferase reporter assay. With the assay, relative estrogenic potency of four selected estrogen receptor (ER) agonists, i.e. diethylstilbestrol, beta-estradiol, 4-nonylphenol and bisphenol A, were determined. Immunoblot analysis revealed that the ER agonists at both EC80 and EC100 caused rapid and transient phosphorylation of extracellular signal-regulated kinases (ERK) in an ER-dependent manner. (more)


Indeno 1,2,3-cd pyrene, a common environmental polycyclic aromatic hydrocarbon, enhances allergic lung inflammation via aryl hydrocarbon receptor
Wong, TH;Lee, CL;Su, HH;Huang, SK;Suen, JL
Abstract:Background: Chronic exposure to ambient polycyclic aromatic hydrocarbons (PAHs) is associated with asthma. Objectives: We aimed to identify the main component of ambient PAHs in southern Taiwan and elucidate their role in allergic asthma. Methods: One-year air samplings with measurement of 16 PAHs were performed.(more)


Development of an efficient viral aerosol collector for higher sampling flow rate
Lin, XT;Hsu, NY;Wang, JR;Chen, NT;Su, HJ;Lin, MY
Abstract:Viral aerosol infection through cough generates large amounts of viral aerosol and can result in many adverse health effects such as influenza flu and severe acute respiratory syndrome (SARS). To characterize the coughed viral aerosol, the sampler needs to sample at higher flow rate and possess high physical collection efficiency as well as high viral preservation. However, most current inertia-based high flow bioaerosol samplers are not suited for viral aerosol sampling since the viability will be lost doing the sampling process.(more)


Risk factors for Asthma Occurrence in children with early-onset atopic dermatitis: An 8-year follow-up study
Huang, CC;Chiang, TL;Chen, PC;Lin, SJ;Wen, HJ;Guo, YL
Abstract:BACKGROUND: Children with early-onset atopic dermatitis (AD) are at substantial risk of developing asthma later in life, and identifying the critical window of detrimental exposure is advantageous for implementing preventive actions. The aim of this study was to evaluate the role of exposure to environmental modifiers during pregnancy and early childhood in asthma occurrence in an infantile AD cohort.(more)


Lifetime exposure to particulate air pollutants is negatively associated with lung function in non-asthmatic children
Tsui, HC;Chen, CH;Wu, YH;Chiang, HC;Chen, BY;Guo, YL
Abstract:BACKGROUND: Pulmonary function is known to be affected by acute and subacute exposure to ambient air pollution. However, the impacts of lifetime exposure to air pollution on the pulmonary function of children have been inconsistent. The present study investigated the impact of lifetime residential exposure to intermediate levels of air pollution on the pulmonary function of schoolchildren. (more)


Prenatal and childhood exposure to phthalate diesters and sex steroid hormones in 2-, 5-, 8-, and 11-year-old children: A pilot study of the Taiwan Maternal and Infant Cohort Study
Wen, HJ;Sie, L;Su, PH;Chuang, CJ;Chen, HY;Sun, CW;Huang, LH;Hsiung, CA;Julie Wang, SL
Abstract:BACKGROUND: Phthalate diesters are commonly used and have been well established as environmental endocrine disruptors. However, few studies have examined their effects on sex steroid hormones in children. We followed children over time to examine the association between pre- and post-natal phthalate exposure and sex steroid hormone levels at 2, 5, 8, and 11 years of age. (more)


Association between fine particulate air pollution and hospital admissions for chest pain in a subtropical city: Taipei, Taiwan
Chen, CC;Yang, CY
Abstract:This study determined the association between fine particles (PM2.5) levels and hospital admissions for chest pain (CP) in Taipei, Taiwan. Hospital admissions for CP and ambient air pollution data for Taipei were obtained for the period 2009-2013. The relative risk of hospital admissions was estimated using a case-crossover approach, after controlling for weather variables, day of the week, seasonality, and long-term time trends. (more)


Upregulation of microRNA-4417 and its target genes contribute to nickel chloride-promoted lung epithelial cell fibrogenesis and tumorigenesis
Wu, CH;Hsiao, YM;Yeh, KT;Tsou, TC;Chen, CY;Wu, MF;Ko, JL
Abstract:Nickel compounds have been classified as carcinogens and shown to be associated with induction of epithelial-mesenchymal transition (EMT) in fibrogenesis and tumorigenesis, as well as the crucial role of microRNAs (miRNAs) and their related genes in controlling EMT and cancer metastasis. Thus, the mechanisms involved in the regulation of EMT in nickel-treated cells are of potential interest in understanding lung fibrosis and tumor progression.(more)


Farming and mortality rates attributed to non–Hodgkin’s lymphoma in Taiwan
Tsai, S;Weng, Y;Chiu, Y;Yang, C
Abstract:Previous studies suggested an elevated risk of non-Hodgkin’s lymphoma (NHL) among farmers. To date, no apparent study examined the association between farming and NHL in Asian countries. The aim of this study was to investigate whether farmers in Taiwan exhibited an increased risk of mortality attributed to NHL. To this end, a mortality odds ratio (MOR) study was conducted to estimate the relative risk of mortality attributed to NHL for farmers in Taiwan.(more)


Stromal C-type lectin receptor COLEC12 integrates H. pylori, PGE2-EP2/4 axis and innate immunity in gastric diseases
Chang, LL;Hsu, WH;Kao, MC;Chou, CC;Lin, CC;Liu, CJ;Weng, BC;Kuo, FC;Kuo, CH;Wang, CJ;Lin, CH;Wu, DC;Huang, SK
Abstract:Background: Tissue stroma is known to be important in regulating Hp-mediated inflammation, but the molecular basis of its interaction with Hp and its potential crosstalk with dendritic cells (DCs) remain to be determined. To this end, the potential crosstalk between H. pylori (Hp) infected gastric stromal cells (Hp-GSCs) and DCs were investigated. Methods: The expression of IL-23 and IL-17 indicative activation of DCs and Th17 cells, respectively, was evaluated as the primary experimental readouts. (more)


Cyclin D1 promoter -56 and -54bp CpG un-methylation predicts invasive progression in arsenic-induced Bowen’s disease
Liao, WT;You, HL;Chai, CY;Lee, CH;Lan, CE;Chang, SJ;Yu, CL;Yu, HS
Abstract:BACKGROUND: Patients with arsenic-induced Bowen’s disease (As-BD) are at risk of developing invasive cancers in the skin, lung, and urinary bladder. However, a longitudinal follow-up study on the association between As-BD and invasive cancers is still lacking. OBJECTIVES: This study aims to investigate the underlying molecular mechanisms of this malignant progression in the skin and internal organs. METHODS: This is a biopsy-based follow-up study. (more)


Berberine activates Aryl Hydrocarbon Receptor but suppresses CYP1A1 induction through miR-21-3p stimulation in MCF-7 breast cancer cells
Lo, SN;Wang, CW;Chen, YS;Huang, CC;Wu, TS;Li, LA;Lee, IJ;Ueng, YF
Abstract:Berberine and the methylenedioxy ring-opening derivatives palmatine and jatrorrhizine are active ingredients in immunomodulatory plants, such as goldenseal. This study aimed to illustrate the effects of protoberberines on aryl hydrocarbon receptor (AhR) activation and cytochrome P450 (CYP) 1 in the estrogen receptor (ER)alpha(+) MCF-7 breast cancer cells. Among protoberberines at non-cytotoxic concentrations (more)


Evidence of high di(2-ethylhexyl) phthalate (DEHP) exposure due to tainted food intake in Taiwanese pregnant women and the health effects on birth outcomes
Tsai, YA;Tsai, MS;Hou, JW;Lin, CL;Chen, CY;Chang, CH;Liao, KW;Wang, SL;Chen, BH;Wu, MT;Hsieh, CJ;Chen, ML;Group, T
Abstract:The contamination of a clouding agent with di(2-ethylhexyl) phthalate (DEHP), a substitute emulsifier-containing compound used in a variety of foods was announced on May 23, 2011. The aims of this study were as follows (1) compare the urine phthalates (PAE) metabolites concentration and estimate the daily intake (DI) of PAEs in pregnant women before and after the tainted food scandal and (2) examine the effect of relatively high PAEs exposure on birth outcome. (more)


Phthalate esters and childhood asthma: A systematic review and congener-specific meta-analysis
Li, MC;Chen, CH;Guo, YL
Abstract:Introduction Exposure to phthalate esters (PAEs) has been associated with childhood asthma, but the congener-specific effects of PAEs on childhood asthma were unclear. We aimed to systematically review and meta-analyze observational studies on the associations between specific effects of PAEs and the risk of childhood asthma. Material and methods Relevant studies were identified by searching three databases up to October 20, 2016. (more)


Gestational diabetes mellitus was related to ambient air pollutant nitric oxide during early gestation
Pan, SC;Huang, CC;Lin, SJ;Chen, BY;Chang, CC;Guo, YL
Abstract:Background Ambient air pollution has been linked to the risk of gestational diabetes mellitus (GDM). However, evidence of this association is limited, and no study has examined the effects of nitric oxide (NO). Objective This study investigated the association between air pollution exposure during gestation and GDM. Methods The Taiwan Birth Cohort Study database was used to examine the association between the risk of GDM and all routinely monitored air pollutants among 21,248 women who were pregnant during 2004–2005. (more)


Prenatal and childhood exposure to phthalate diesters and thyroid function in a 9-year follow-up birth cohort study: Taiwan maternal and infant cohort study
Huang, HB;Chuang, CJ;Su, PH;Sun, CW;Wang, CJ;Wu, MT;Wang, SL
Abstract:BACKGROUND: Phthalates are widely used in industry, personal care products, and medications. Recent studies have suggested that phthalate exposure alters thyroid hormones. However, longitudinal studies concerning the association between phthalate exposure and thyroid function in children are scant. Therefore, we examined the association between pre- and postnatal phthalate exposure and thyroid function in children born in 2000-2001. METHODS: We studied 181 mother-child pairs in central Taiwan and followed-up the children from 2000 to 2009 at 2, 5, and 8 years old. (more)


Birth Cohort Consortium of Asia: Current and future perspectives
Kishi, R;Zhang, JJ;Ha, EH;Chen, PC;Tian, Y;Xia, Y;Tsuchiya, KJ;Nakai, K;Kim, S;Hong, SJ;Hong, YC;Lee, JR;Jan Mohamed, HJB;Parajuli, RP;Adair, LS;Chong, YS;Guo, YL;Wang, SL;Nishijo, M;Kido, T;Tai, PT;Nandasena, S
Abstract:BACKGROUND: The environmental health of children is one of the great global health concerns. Exposures in utero and throughout development can have major consequences on later health. However, environmental risks or disease burdens vary from region to region. Birth cohort studies are ideal for investigating different environmental risks. (more)


Use of genetic algorithm combinational single-nucleotide polymorphisms could modify the association of blood lead levels and bone matrix density
Chen, CJ;Chang, HW;Yang, CH;Chuang, HY;Yu, HS
Abstract:BACKGROUND: Previous studies have not explored the relationship between a single gene and a single disease. Our study aims to investigate the association of multiple genotypes with blood lead levels and bone matrix density (BMD) by using genetic algorithms. METHODS: Our research focused on 506 employees (245 males and 261 females) of a lead battery factory in Taiwan. (more)


A composite of exhaled LTB4 , LXA4 , FeNO and FEV1 as an “asthma classification ratio” characterizes childhood asthma
Chen, LC;Tseng, HM;Kuo, ML;Chiu, CY;Liao, SL;Su, KW;Tsai, MH;Hua, MC;Lai, SH;Yao, TC;Yeh, KW;Wu, AH;Huang, JL;Huang, SK
Abstract:BACKGROUND: Aberrant generation of eicosanoids is associated with asthma, but the evidence remains incomplete and its potential utility as biomarkers is unclear. Major eicosanoids in exhaled breath condensates (EBCs) were assessed as candidate markers for childhood asthma. METHODS: Ten exhaled eicosanoid species was evaluated using ELISA in the discovery phase, followed by prediction model-building and validation phases. (more)


Association between emotional symptoms and job demands in an Asian electronics factory
Huang, WL;Guo, YL;Chen, PC;Wang, J;Chu, PC
Abstract:Various work-related issues including mental health have been described for the electronic industry. Although East Asian countries play important roles in the electronics industry, the association between job demands and emotional symptoms has been rarely examined. The present study recruited 603 workers from either office or clean room environments in an electronics factory in Taiwan. Their personal factors, work-related factors, and emotional symptoms were assessed by a self-administered questionnaire. (more)


PM2.5 components and outpatient visits for asthma: A time-stratified case-crossover study in a suburban area
Jung, CR;Young, LH;Hsu, HT;Lin, MY;Chen, YC;Hwang, BF;Tsai, PJ
Abstract:The effects of fine particles (PM2.5) on asthma have been widely confirmed by epidemiological research studies. However, a limited number of studies have investigated the relationship between exposure to different PM2.5 components and asthma. We characterized the PM2.5 components in a suburban site of central Taiwan and conducted a time-stratified case-crossover study to elaborate the effects of daily concentration of each PM2.5 component on asthma outpatient visits. (more)


Effects of a national smoking ban on hospital admissions for cardiovascular diseases: A time-series analysis in Taiwan
Yang, YN;Huang, YT;Yang, CY
Abstract:On January 11, 2009, a comprehensive smoking ban was implemented in Taiwan. The aim of this study was to evaluate the effect of this ban on hospital admissions for ischemic heart disease (IHD). Trends in the country-level monthly hospital admission rates for IHD were determined and frequency compared to other conditions such as control conditions cholecystitis, bowel obstruction, and appendicitis from January 1997 (1 year before the first phase of smoke-free laws was implemented) to December 2012 (3 years after the second phase of the ban). (more)


Depressive, anxiety and post-traumatic stress disorders at six years after occupational injuries
Chin, WS;Shiao, JSC;Liao, SC;Kuo, CY;Chen, CC;Guo, YL
Abstract:The aim of this study is to determine the prevalence rates of depressive, anxiety and PTSDs, and the risk factors for psychological symptoms at 6 years after occupational injury. This longitudinal study followed workers who were occupationally injured in 2009. Psychological symptoms and return to work were assessed at 3 and 12 months after injury. Injured workers who had completed the initial questionnaire survey at 3 or 12 months after injury were recruited. (more)


Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase extraction stable-isotope dilution liquid chromatography-tandem mass spectrometry
Chen, CY;Lin, P;Tsai, MH;Lee, HL
Abstract:Lipidomics is the area of study dedicated to the comprehensive analysis and characterization of the functions and metabolism of lipids in biological samples. One of the most comprehensively studied classes of lipids is polyunsaturated fatty acids (PUFAs). Eicosanoids are a series of bioactive lipid mediators that are metabolized from PUFAs and generated majorly from the precursor arachidonic acid. This study identified the profiles of target eicosanoids after acute exposure to arsenic. (more)


The decline in kidney function with chromium exposure is exacerbated with co-exposure to lead and cadmium
Tsai, TL;Kuo, CC;Pan, WH;Chung, YT;Chen, CY;Wu, TN;Wang, SL
Abstract:Environmental factors contribute significantly to the pathogenesis of chronic kidney disease. However, these factors, and particularly the toxic effects of heavy metals, have not been completely evaluated. Chromium is a widespread industrial contaminant that has been linked to nephrotoxicity in animal and occupational population studies. Nevertheless, its role in population renal health and its potential interactions with other nephrotoxic metals, such as lead and cadmium, remain unknown. (more)


Estrogen and cigarette sidestream smoke particulate matter exhibit ERalpha-dependent tumor-promoting effects in lung adenocarcinoma cells
Kuo, LC;Cheng, LC;Lee, CH;Lin, CJ;Chen, PY;Li, LA
Abstract:BACKGROUND: Estrogen and secondhand smoke are key risk factors for nonsmoking female lung cancer patients, who frequently have lung adenocarcinoma and show tumor ERalpha expression. We speculated that estrogen and secondhand smoke might cause harmful effects via ERalpha signaling. Our results showed that 17beta-estradiol (E2), the primary form of endogenous estrogen, exacerbated proliferation, migration, and granzyme B resistance of lung adenocarcinoma cells in an ERalpha-dependent manner. (more)


Heat wave and mortality: A multicountry, multicommunity study
Guo, Y;Gasparrini, A;Armstrong, BG;Tawatsupa, B;Tobias, A;Lavigne, E;Coelho, M;Pan, X;Kim, H;Hashizume, M;Honda, Y;Guo, YL;Wu, CF;Zanobetti, A;Schwartz, JD;Bell, ML;Scortichini, M;Michelozzi, P;Punnasiri, K;Li, S;Tian, L;Garcia, SDO;Seposo, X;Overcenco, A;Zeka, A;Goodman, P;Dang, TN;Dung, DV;Mayvaneh, F;Saldiva, PHN;Williams, G;Tong, S
Abstract:BACKGROUND: Few studies have examined variation in the associations between heat waves and mortality in an international context. OBJECTIVES: We aimed to systematically examine the impacts of heat waves on mortality with lag effects internationally. METHODS: We collected daily data of temperature and mortality from 400 communities in 18 countries/regions and defined 12 types of heat waves by combining community-specific daily mean temperature >/=90th, 92.5th, 95th, and 97.5th percentiles of temperature with duration >/=2, 3, and 4 d.(more)


The association of arsenic metabolism with cancer, cardiovascular disease, and diabetes: A systematic review of the epidemiological evidence
Kuo, CC;Moon, KA;Wang, SL;Silbergeld, E;Navas-Acien, A
Abstract:BACKGROUND: The available evidence on the role of arsenic metabolism in individual susceptibility to the development of cancer, cardiovascular disease, and diabetes has not been formally and comprehensively reviewed. OBJECTIVES: Our goal was to systematically investigate the association of arsenic metabolism with cancer, cardiovascular disease, and diabetes-related outcomes in epidemiologic studies. As a secondary objective, we characterized the variation of arsenic metabolism in different populations worldwide.(more)


Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer
Wang, LT;Chiou, SS;Chai, CY;Hsi, E;Chiang, CM;Huang, SK;Wang, SN;Yokoyama, KK;Hsu, SH
Abstract:The epithelial-mesenchymal transition (EMT) is an important process in the progression of cancer. However, its occurrence and mechanism of regulation are not fully understood. We propose a regulatory pathway in which spermatogenic leucine zipper 1 (SPZ1) promotes EMT through its transactivating ability in increasing TWIST1 expression. We compared the expression of SPZ1 and TWIST1 in specimens of hepatocarcinoma cells (HCCs) and non-HCCs.(more)


Intestine-specific homeobox gene ISX Integrates IL6 signaling, tryptophan catabolism, and immune suppression
Wang, LT;Chiou, SS;Chai, CY;Hsi, E;Yokoyama, KK;Wang, SN;Huang, SK;Hsu, SH
Abstract:The intestine-specific homeobox transcription factor ISX is an IL-6-inducible proto-oncogene implicated in the development of hepatocellular carcinoma (HCC), but its mechanistic contributions to this process are undefined. In this study, we provide evidence that ISX mediates a positive feedback loop integrating inflammation, tryptophan cataboism and immune suppression. We found that ISX mediated IL-6-induced expression of the tryptophan catabolic enzymes IDO1 and TDO2 in HCC cells, resulting in an ISX-dependent increase in the tryptophan catabolite kynurenine and its receptor aryl hydrocarbon receptor (AHR).(more)


Ambient fungal spore concentration in a subtropical metropolis: Temporal distributions and meteorological determinants
Kallawicha, K;Chen, YC;Chao, HJ;Shen, WC;Chen, BY;Chuang, YC;Guo, YL
Abstract:Ambient particles comprise approximately 25% of fungal spores, which cause adverse health outcomes such as respiratory diseases, allergy, and infection. In this study, we investigated temporal variations and distributions of ambient fungal spores in an urban area of the Taipei metropolis for over 1 year. A Burkard 7-day volumetric spore trap was used to collect air samples. Samples collected daily were stained, counted, and identified on the basis of morphological characteristics. The associations between fungal spores and environmental parameters were then evaluated through multiple regression analysis. (more)


Association of time-serial changes in ambient particulate matters (PMs) with respiratory emergency cases in Taipei’s Wenshan District
Chang, JH;Hsu, SC;Bai, KJ;Huang, SK;Hsu, CW
Abstract:Ambient air pollution poses a significant risk for a group of common and often debilitating respiratory diseases, but its direct impact on cause-specific respiratory diseases using emergency room visit (ERV) as an indicator remains to be fully explored. In this study, we conducted a time-series study of ambient PM2.5, NO2, SO2 and their association with ERV for asthma, COPD and pneumonia in a four-year time span. Relative risks for ERV as per log increase in the level of ambient pollutants with time lags of up to 10 days were calculated, using a generalized additive model of Poisson regression. Daily 24-h average concentrations of PM2.5 and pollutant gases were obtained from a local Gutting air quality monitoring station.(more)


Ambient PM2.5 in the residential area near industrial complexes: Spatiotemporal variation, source apportionment, and health impact
Hsu, CY;Chiang, HC;Chen, MJ;Chuang, CY;Tsen, CM;Fang, GC;Tsai, YI;Chen, NT;Lin, TY;Lin, SL;Chen, YC
Abstract:This study systemically investigated the ambient PM2.5 (n=108) with comprehensive analyses of the chemical composition, identification of the potential contributors, and estimation of the resultant respiratory physician visits in the residential regions near energy-consuming and high-polluting industries in central Taiwan. The positive matrix fraction (PMF) model with chemical profiles of trace metals, water-soluble ions, and organic/elemental carbons (OC/EC) was applied to quantify the potential sources of PM2.5. (more)


Functional mri imaging signatures in young men with prenatal exposure to dioxin-like chemicals
Guo, L;Chu, CP;Huang, YC;Hsieh, ST
Abstract:Introduction: In 1979, an episode of food intoxication by rice oil contaminated with dioxin-like chemicals occurred in Taiwan called Yucheng (oil-syndrome in Chinese). The children born to exposed women suffered from skin manifestations, tooth problems, reduced neurocognitive capability, etc. We aim to determine whether children born to Yucheng mothers had changes in functional MRI imaging signatures. Methods: The fMRI study was performed using a 3T MRI scanner. Gradient-echo echo-planar imaging (EPI) was used to acquire BOLD signal. Two different paradigms for fMRI, N-back task and picture rotation task, were used to simulate coding and object assembly subtest. All data were processed using SPM8(www.fil.ion.ucl.ac.uk/spm) implemented on MATLAB.(more)


Intellectual evaluation of children exposed to phthalate-tainted products after the 2011 Taiwan phthalate episode
Huang, PC;Tsai, CH;Chen, CC;Wu, MT;Chen, ML;Wang, SL;Chen, BH;Lee, CC;Jaakkola, JJK;Wu, WC;Chen, MK;Hsiung, CA;Group, R
Abstract:Phthalate exposure may reduce intellectual development in young children. In 2011, numerous Taiwanese children had been reported to have consumed phthalate-tainted products. We investigated the effects of phthalate exposure on the intellectual development of these children after the 2011 Taiwan di-2-ethylhexyl phthalate (DEHP) episode. Methods We recruited 204 children, aged 3–12 y, from 3 hospitals in Taiwan between 2012 and 2013. First-morning urine samples were collected for analyzing 5 phthalate metabolites. We applied a Bayesian model to estimate the past DEHP exposure (estDEHPADD) of each participant before the 2011 DEHP episode.(more)


Exposure sources and their relative contributions to urinary phthalate metabolites among children in Taiwan
Chen, CC;Wang, YH;Wang, SL;Huang, PC;Chuang, SC;Chen, MH;Chen, BH;Sun, CW;Fu, HC;Lee, CC;Wu, MT;Chen, ML;Hsiung, CA
Abstract:Phthalate exposure is omnipresent and known to have developmental and reproductive effects in children. The aim of this study was to determine the phthalate exposure sources and their relative contributions among children in Taiwan. During the first wave of the Risk Assessment of Phthalate Incident in Taiwan (RAPIT), in 2012, we measured 8 urinary phthalate metabolites in 226 children aged 1–11 years old and in 181 children from the same cohort for the wave 2 study in 2014. A two-stage statistical analysis approach was adopted. (more)


Mannose receptor modulates macrophage polarization and allergic inflammation through miR-511-3p
Zhou, Y;Do, DC;Ishmael, FT;Squadrito, ML;Tang, HM;Tang, HL;Hsu, MH;Qiu, L;Li, C;Zhang, Y;Becker, KG;Wan, M;Huang, SK;Gao, P
Abstract:BACKGROUND: Mannose receptor (MRC1/CD206) has been suggested to mediate allergic sensitization and asthma to multiple glyco-allergens, including cockroach allergens. OBJECTIVE: Determine the existence of a protective mechanism through which MRC1 limits allergic inflammation through its intronic miR-511-3p. METHODS: We examined the MRC1-mediated cockroach allergen uptake by lung macrophages and lung inflammation using C57BL/6 wild-type (WT) and Mrc1-/- mice.(more)


Hydrogen gas protects IP3Rs by reducing disulfide bridges in human keratinocytes under oxidative stress
Wu, CY;Hsu, WL;Tsai, MH;Liang, JL;Lu, JH;Yen, CJ;Yu, HS;Noda, M;Lu, CY;Chen, CH;Yan, SJ;Yoshioka, T
Abstract:Based on the oxidative stress theory, aging derives from the accumulation of oxidized proteins induced by reactive oxygen species (ROS) in the cytoplasm. Hydrogen peroxide (H2O2) elicits ROS that induces skin aging through oxidation of proteins, forming disulfide bridges with cysteine or methionine sulfhydryl groups. Decreased Ca2+ signaling is observed in aged cells, probably secondary to the formation of disulfide bonds among Ca2+ signaling-related proteins. Skin aging processes are modeled by treating keratinocytes with H2O2.(more)


Short-term effects of ozone air pollution on hospital admissions for myocardial infarction: A time-stratified case-crossover study in Taipei
Chiu, H;Weng, Y;Chiu, Y;Yang, C
Abstract:This study was undertaken to determine whether there was a correlation between ambient ozone (O3) levels and number of hospital admissions for myocardial infarction (MI) in Taipei, Taiwan. Hospital admissions for MI and ambient air pollution data for Taipei were obtained for the period from 2006 to 2010. The relative risk (RR) of hospital admissions for MI was estimated using a time-stratified case-crossover approach, controlling for weather variables, day of the week, seasonality, and long-term time trends. For the single-pollutant model (without adjustment for other pollutants), increased RR for a number of MI admissions was significantly associated with higher O3 levels both on warm days (>23°C) and on cool days (<23°C). (more)


Short-term effects of fine particulate air pollution on hospital admissions for hypertension: A time-stratified case-crossover study in Taipei
Chiu, HF;Tsai, SS;Yang, CY
Abstract:This study was undertaken to determine whether there was a correlation between fine particle (PM2.5) levels and hospital admissions for hypertension in Taipei, Taiwan. Hospital admissions for hypertension and ambient air pollution data for Taipei were obtained for the period from 2009 to 2013. The relative risk of hospital admissions was estimated using a case-crossover approach, controlling for weather variables, day of the week, seasonality, and long-term time trends. For the single pollutant model (without adjustment for other pollutants), the risk of hospital admissions for hypertension was estimated to increase by 12% on warm days (>23 degrees C) and 2% on cool days (<23 degrees C), respectively.(more)


Global DNA methylation and oxidative stress biomarkers in workers exposed to metal oxide nanoparticles
Liou, SH;Wu, WT;Liao, HY;Chen, CY;Tsai, CY;Jung, WT;Lee, HL
Abstract:This is the first study to assess global methylation, oxidative DNA damage, and lipid peroxidation in workers with occupational exposure to metal oxide nanomaterials (NMs). Urinary and white blood cell (WBC) 8-hydroxydeoxyguanosine (8-OHdG), and exhaled breath condensate (EBC) 8-isoprostane were measured as oxidative stress biomarkers. WBC global methylation was measured as an epigenetic alteration. Exposure to TiO2, SiO2, and indium tin oxide (ITO) resulted in significantly higher oxidative biomarkers such as urinary 8-OHdG and EBC 8-isoprostane. (more)


Interleukin-24 as a target cytokine of environmental aryl hydrocarbon receptor agonist exposure in the lung
Luo, YH;Kuo, YC;Tsai, MH;Ho, CC;Tsai, HT;Hsu, C;Chen, YC;Lin, P
Abstract: Exposure to environmental aryl hydrocarbon receptor (AhR) agonists, such as halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons (PAHs), has great impacts on the development of various lung diseases. As emerging molecular targets for AhR agonists, cytokines may contribute to the inflammatory or immunotoxic effects of environmental AhR agonists. However, general cytokine expression may not specifically indicate environmental AhR agonist exposure. (more)


Sitagliptin may reduce breast cancer risk in women with type 2 Diabetes
Tseng, CH
Abstract:BACKGROUND: Whether sitagliptin may affect breast cancer risk remains to be answered. This study evaluated such an association in Taiwanese female patients with type 2 diabetes. METHODS: A retrospective cohort of female patients with newly diagnosed type 2 diabetes at an age >/= 25 years between 1999 and 2010 was recruited from the National Health Insurance database. A total of 32,457 ever-users and 396,021 never-users of sitagliptin were followed until December 31, 2011.(more)


An integrative transcriptomic analysis reveals bisphenol A exposure-induced dysregulation of microRNA expression in human endometrial cells
Chou, WC;Lee, PH;Tan, YY;Lin, HC;Yang, CW;Chen, KH;Chuang, CY
Abstract:Bisphenol A (BPA) are commonly used in the manufacture of polycarbonate plastics. Higher BPA exposure levels have been found in patients with endometrial hyperplasia that is one of risk factors of endometrial cancer (EC). Aberrant microRNAs (miRNAs) regulation has been observed in the development of cancer. Thus, this study investigated whether BPA exposure can disrupt miRNA regulation and its gene expression regarding to EC carcinogenic progress. Microarray experiments of miRNA and mRNA were performed in human endometrial cancer RL95-2 cells with treatment of low-to-moderate (10, 103 and 105nM) BPA to explore the aberrant genes corresponding to human EC progression.(more)


IL-17F induces IL-6 via TAK1-NFkappaB pathway in airway smooth muscle cells
Nakajima, M;Kawaguchi, M;Ota, K;Fujita, J;Matsukura, S;Huang, S-K;Morishima, Y;Ishii, Y;Satoh, H;Sakamoto, T;Hizawa, N
Abstract:Introduction Interleukin (IL)-17F plays a critical role in the pathophysiology of asthma. However, the precise role of IL-17F in airway smooth muscle cells (ASMCs) and its regulatory mechanisms remain to be defined. Therefore, we sought to investigate the expression of IL-6 by IL-17F and the involvement of transforming growth factor β-activated kinase 1 (TAK1) and nuclear factor (NF)-κB by in ASMCs. Methods ASMCs were cultured in the presence or absence of IL-17F. The expression of IL-6 gene and protein was analyzed using real-time PCR and ELISA, and the activation of TAK1 and NF-κB was detected by Western blotting. The effect of TAK1 inhibitor 5Z-7-oxozeaenol and NF-κB inhibitor BAY 11-7082 on the expression of IL-6 was investigated.(more)


Mixture risk assessment due to ingestion of arsenic, copper, and zinc from milkfish farmed in contaminated coastal areas
Lin, YJ;Ling, MP;Chen, SC;Chen, WY;Hsieh, NH;Cheng, YH;You, SH;Chou, WC;Lin, MC;Liao, CM
Abstract:Human health risks associated with the consumption of metal-contaminated fish over extended periods have become a concern particularly in Taiwan, where fish is consumed on a large scale. This study applied the interaction-based hazard index (HI) to assess the mixture health risks for fishers and non-fishers who consume the arsenic (As), copper (Cu), and zinc (Zn) contaminated milkfish from As-contaminated coastal areas in Taiwan, taking into account joint toxic actions and potential toxic interactions.(more)


Metformin and lung cancer risk in patients with type 2 diabetes mellitus
Tseng, CH
Abstract:This study evaluated whether metformin might reduce lung cancer risk. The reimbursement database of the Taiwan’s National Health Insurance was used. A sample of 15414 never users and 280159 ever users of metformin (original sample) and a 1:1 matched-pairs of ever and never users (n=15414 in each group, matched sample) were recruited from patients with newly diagnosed type 2 diabetes mellitus during 1999-2005. They were followed until December 31, 2011.(more)


Genome-wide DNA methylation at birth in relation to in utero arsenic exposure and the associated health in later life
Kaushal, A;Zhang, H;Karmaus, WJJ;Everson, TM;Marsit, CJ;Karagas, MR;Tsai, SF;Wen, HJ;Wang, SL
Abstract:BACKGROUND: In utero arsenic exposure may alter fetal developmental programming by altering DNA methylation, which may result in a higher risk of disease in later life. We evaluated the association between in utero arsenic exposure and DNA methylation (DNAm) in cord blood and its influence in later life. METHODS: Genome-wide DNA methylation in cord blood from 64 subjects in the Taiwanese maternal infant and birth cohort was analyzed.(more)


A histone deacetylase inhibitor enhances expression of genes inhibiting Wnt pathway and augments activity of DNA demethylation reagent against non-small cell lung cancer
Shieh, JM;Tang, YA;Hu, FH;Huang, WJ;Wang, YJ
Abstract:Development of new inhibitors targeting histone deacetylases (HDACs) with improved efficacy for solid tumor therapy is urgently needed. Here, we report the development of a novel HDAC inhibitor TMU-35435 and verify it as a single agent and in combination treatment with DNA demethylation reagent 5-aza-2′-deoxycytidine (5-aza-dC) in lung cancer pre-clinical models. TMU-35435 exerted cancer-specific cytotoxicity via mitochondria-mediated apoptosis. (more)


Estimating lifetime medical costs from censored claims data
Hwang, JS;Hu, TH;Lee, LJH;Wang, JD
Abstract:Claims databases consisting of routinely collected longitudinal records of medical expenditures are increasingly utilized for estimating expected medical costs of patients with a specific condition. Survival data of the patients of interest are usually highly censored, and observed expenditures are incomplete. In this study, we propose a survival-adjusted estimator for estimating mean lifetime costs, which integrates the product of the survival function and the mean cost function over the lifetime horizon. (more)


Environmental tobacco smoke: Relationship to early pregnancy discomforts
Hung, HJ;Chen, CY;Wang, SL;Wu, TN;Lee, CH;Cheng, SY
Abstract: OBJECTIVES: We assessed environmental tobacco smoke (ETS) and examined its association with pregnancy discomforts. METHODS: We used structured questionnaires to interview a convenience sample of 139 pregnant women (8-20 weeks of gestation) recruited from 2 hospitals in central Taiwan. RESULTS: We found that 84% of the participants experienced ETS exposure in their households, workplaces, and/or public areas. Bivariate analyses showed the severity of pregnancy discomforts in the participants exposed to a high level of ETS was higher than that in those exposed to a low level of ETS.(more)


Severe aortic arch calcification predicts mortality in patients undergoing peritoneal dialysis
Wu, CF;Lee, YF;Lee, WJ;Su, CT;Lee, LJ;Wu, KD;Chen, PC;Kao, TW
Abstract:BACKGROUND/PURPOSE: Vascular calcification can predict cardiovascular (CV) morbidity and mortality in patients with end-stage renal disease. We evaluated the prevalence, association factors, and outcomes of chest X-ray-detected aortic arch calcification (AoAC) in patients undergoing peritoneal dialysis (PD). METHODS: We included 190 patients undergoing PD (mean age, 52.6 +/- 14.3 years) for whom chest radiographs were available. AoAC revealed by chest X-ray was graded from 0 to 3 according to an AoAC score (AoACS).(more)


Land-use regression with long-term satellite-based greenness index and culture-specific sources to model PM2.5 spatial-temporal variability
Wu, CD;Chen, YC;Pan, WC;Zeng, YT;Chen, MJ;Guo, YL;Lung, SC
Abstract:This study utilized a long-term satellite-based vegetation index, and considered culture-specific emission sources (temples and Chinese restaurants) with Land-use Regression (LUR) modelling to estimate the spatial-temporal variability of PM2.5 using data from Taipei metropolis, which exhibits typical Asian city characteristics. Annual average PM2.5 concentrations from 2006 to 2012 of 17 air quality monitoring stations established by Environmental Protection Administration of Taiwan were used for model development. PM2.5 measurements from 2013 were used for external data verification.(more)


Urban air pollution and meteorological factors affect emergency department visits of elderly patients with chronic obstructive pulmonary disease in Taiwan
Ding, PH;Wang, GS;Guo, YL;Chang, SC;Wan, GH
Abstract:Both air pollution and meteorological factors in metropolitan areas increased emergency department (ED) visits from people with chronic obstructive pulmonary disease (COPD). Few studies investigated the associations between air pollution, meteorological factors, and COPD-related health disorders in Asian countries. This study aimed to investigate the relationship between the environmental factors and COPD-associated ED visits of susceptible elderly population in the largest Taiwanese metropolitan area (Taipei area, including Taipei city and New Taipei city) between 2000 and 2013.(more)


Differential immunological effects of infrared irradiation and its associated heat in vivo
Lee, CH;Hong, CH;Liao, WT;Yu, HS
Abstract:Infrared irradiation (IR) is the most abundant fraction of sunlight reaching the earth’s surface and provides heat. The fever response of an animal is known to regulate its immune responses. However, the non-thermal immune responses of IR were difficult to assess owing to its close association with heat. We hypothesized that IR irradiation induced differential immunological responses, independent of its associated heat. With an IR machine coupled with a delicate temperature control system, we investigated the non-thermal immunological effects of IR in vivo. (more)


Benzopyrene mobilizes Langerhans cells and polarizes Th2/17 responses in epicutaneous protein sensitization via aryl hydrocarbon receptor
Hong, CH;Lee, CH;Yu, HS;Huang, SK
Abstract:Atopic dermatitis (AD) is a common disease with genetic and environmental interactions. We previously reported lifetime exposure to cigarette smoke is associated with adult-onset AD. Aryl hydrocarbon receptor (AhR) is important in regulating environmental exposure to xenobiotics, including benzopyrenes (BP), a major polycyclic aromatic hydrocarbon (PAH) present in cigarette smoke. However, how AhR regulates immune responses in sensitization phase of AD remained elusive. (more)


Ultraviolet B (UVB) induces development of early melanocytic progenitors via increased oxidative stress in vitro suggesting the use of antioxidants after regimentation in UVB phototherapy for vitiligo
Yu, S;Yu, HS;Lai, HC;Wu, CS;Jheng, HY;Lan, CC

Abstract:Narrowband ultraviolet B (UVB) is the phototherapy of choice for vitiligo. As follicular pigmentation is observed in narrowband UVB phototherapy, repigmentation of vitiligo requires activation of melanocyte stem cells residing in the bulge area of hair follicle, followed by their proper functional development. Our previous research showed activation of aryl hydrocarbon receptor (AhR) signaling and nuclear translocation of epidermal growth factor receptor (EGFR) are involved in UVB-induced differentiation of late stage melanocyte precursors [1].(more)


Exposure to fine particulate matter causes oxidative and methylated DNA damage in young adults: A longitudinal study
Lai, CH;Huang, HB;Chang, YC;Su, TY;Wang, YC;Wang, GC;Chen, JE;Tang, CS;Wu, TN;Liou, SH
Abstract: An increased understanding is needed of the physiological effects and plausible biological mechanisms that link PM2.5 (particulate matter with an aerodynamic diameter below 2.5mum) exposure to mortality and morbidities such as atherosclerosis and respiratory disease. PM2.5 causes carcinogenic health effects. Biomonitoring in humans has suggested that 8-oxo-7, 8-dihydro-2-deoxyguanosine (8-oxodG) and N7-methylguanine (N7-MeG) are correlated with oxidative and methylated DNA damage. (more)


Exposure sources and their relative contributions to urinary phthalate metabolites among children in Taiwan
Chen, CC;Wang, YH;Wang, SL;Huang, PC;Chuang, SC;Chen, MH;Chen, BH;Sun, CW;Fu, HC;Lee, CC;Wu, MT;Chen, ML;Hsiung, CA
Abstract: Phthalate exposure is omnipresent and known to have developmental and reproductive effects in children. The aim of this study was to determine the phthalate exposure sources and their relative contributions among children in Taiwan. During the first wave of the Risk Assessment of Phthalate Incident in Taiwan (RAPIT), in 2012, we measured 8 urinary phthalate metabolites in 226 children aged 1–11 years old and in 181 children from the same cohort for the wave 2 study in 2014. (more)


Phthalate exposure and reproductive hormones and sex-hormone binding globulin before puberty – Phthalate contaminated-foodstuff episode in Taiwan
Wen, HJ;Chen, CC;Wu, MT;Chen, ML;Sun, CW;Wu, WC;Huang, IW;Huang, PC;Yu, TY;Hsiung, CA;Wang, SL;RAPIT group.
Abstract: BACKGROUND: In May 2011, a major incident involving phthalates-contaminated foodstuffs occurred in Taiwan. Di-(2-ethylhexyl) phthalate (DEHP) was added to foodstuffs, mainly juice, jelly, tea, sports drink, and dietary supplements. Concerns arose that normal pubertal development, especially reproductive hormone regulation in children, could be disrupted by DEHP exposure. OBJECTIVE: To investigate the association between phthalate exposure and reproductive hormone levels among children following potential exposure to phthalate-tainted foodstuffs. (more)


Comparison of different cell type correction methods for genome-scale epigenetics studies
Kaushal, A;Zhang, H;Karmaus, WJJ;Ray, M;Torres, MA;Smith, AK;Wang, SL
Abstract: BACKGROUND: Whole blood is frequently utilized in genome-wide association studies of DNA methylation patterns in relation to environmental exposures or clinical outcomes. These associations can be confounded by cellular heterogeneity. Algorithms have been developed to measure or adjust for this heterogeneity, and some have been compared in the literature. However, with new methods available, it is unknown whether the findings will be consistent, if not which method(s) perform better. (more)


Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer
Wang, LT;Chiou, SS;Chai, CY;Hsi, E;Chiang, CM;Huang, SK;Wang, SN;Yokoyama, KK;Hsu, SH
Abstract: The epithelial-mesenchymal transition (EMT) is an important process in the progression of cancer. However, its occurrence and mechanism of regulation are not fully understood. We propose a regulatory pathway in which spermatogenic leucine zipper 1 (SPZ1) promotes EMT through its transactivating ability in increasing TWIST1 expression. We compared the expression of SPZ1 and TWIST1 in specimens of hepatocarcinoma cells (HCCs) and non-HCCs. (more)


SHP-2 phosphatase controls aryl hydrocarbon receptor-mediated ER stress response in mast cells
Wang, HC;Zhou, Y;Huang, SK
Abstract:Previously we reported that exposure of mouse and human mast cells to aryl hydrocarbon receptor (AhR) ligands resulted in reactive oxygen species (ROS)- and calcium (Ca2+)-dependent activation of mast cells in vitro and in vivo. However, the mechanisms through which the AhR-ligand axis mediates stress response, Ca2+ signaling and subsequent mast cell activation remain to be fully elucidated. Evidence is provided herein that SHP-2 is critical in regulating AhR-mediated ER stress response and intracellular Ca2+ dynamics. (more)


Epigenetic synergism between interleukin-4 and aryl-hydrocarbon receptor in human macrophages
Liao, WT;Lu, JH;Wang, WT;Hung, CH;Sheu, CC;Huang, SK
Abstract: The aryl hydrocarbon receptor (AhR)-ligand axis is involved in immune regulation, but its molecular basis remains to be fully elucidated. Chemokine (C-C motif) ligand 1 (CCL1) is an important chemoattractant, but how CCL1 is regulated remains to be defined. The role of AhR in regulating CCL1 expression in two major subsets of macrophage was investigated.(more)


Concentrations and analysis of health risks of ambient air metallic elements at Longjing site in central Taiwan
Fang, GC;Chen, YC;Lo, CT;Cho, MH;Zhuang, YJ;Tsai, KH;Huang, CY;Xiao, YF
Abstract:The concentrations of particulates and metallic elements that were bound to total suspended particulates in ambient air at Long Cyuan Elementary School (LCYES), Lung Ching Elementary School (LCHES) and Long Shan Primary School (LSPS) sampling sites in the Longjing area were measured. Significant difference tests were conducted at LSPS, LCYES and LCHES sites.(more)


Involvement of the cytokine–IDO1–AhR loop in zinc oxide nanoparticle-induced acute pulmonary inflammation
Ho, CC;Lee, HL;Chen, CY;Luo, YH;Tsai, MH;Tsai, HT;Lin, P
Abstract: Zinc oxide nanoparticles (ZnONPs) are widely used in our daily life, such as in sunscreens and electronic nanodevices. However, pulmonary exposure to ZnONPs causes acute pulmonary inflammation, which is considered as an initial event for various respiratory diseases. Thus, elucidation of the underlying cellular mechanisms of ZnONPs can help us in predicting their potential effects in respiratory diseases. (more)


IL-17F induces IL-6 via TAK1-NFkappaB pathway in airway smooth muscle cells
Nakajima, M;Kawaguchi, M;Ota, K;Fujita, J;Matsukura, S;Huang, S-K;Morishima, Y;Ishii, Y;Satoh, H;Sakamoto, T;Hizawa, N
Abstract:Introduction Interleukin (IL)-17F plays a critical role in the pathophysiology of asthma. However, the precise role of IL-17F in airway smooth muscle cells (ASMCs) and its regulatory mechanisms remain to be defined. Therefore, we sought to investigate the expression of IL-6 by IL-17F and the involvement of transforming growth factor β-activated kinase 1 (TAK1) and nuclear factor (NF)-κB by in ASMCs.(more)


Following changes in the axillary secretions of two patients before and after bromhidrosis surgery using liquid chromatography-mass spectrometry
Ho, WT;Lee, LJ;Pan, JY
Abstract: Bromhidrosis negatively affects quality of life, and many patients undergo surgery for treatment. Complete apocrine gland removal markedly reduces body malodor; however, few studies have objectively evaluated changes in apocrine secretions and the degrees of improvement after surgery. We aimed to evaluate these physiological changes in patients who were satisfied after surgery. (more)


Estimated daily intake and cumulative risk assessment of phthalates in the general Taiwanese after the 2011 DEHP food scandal
Chang, JW;Lee, CC;Pan, WH;Chou, WC;Huang, HB;Chiang, HC;Huang, PC
Abstract:A food scandal occurred in Taiwan in 2011 because the DEHP (di-2-ethylhexyl phthalate) had been intentionally used in food products. We assessed the daily intakes (DIs) and cumulative risk of phthalates in Taiwan’s general population after the scandal. The DIs of 6 phthalates, including di-n-butyl phthalate (DnBP), di-iso-butyl phthalate (DiBP), and DEHP, were evaluated using urinary phthalate metabolites. (more)


Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase extraction stable-isotope dilution liquid chromatography-tandem mass spectrometry
Chen, CY;Lin, P;Tsai, MH;Lee, HL
Abstract: Lipidomics is the area of study dedicated to the comprehensive analysis and characterization of the functions and metabolism of lipids in biological samples. One of the most comprehensively studied classes of lipids is polyunsaturated fatty acids (PUFAs). Eicosanoids are a series of bioactive lipid mediators that are metabolized from PUFAs and generated majorly from the precursor arachidonic acid. (more)


Case study III: The construction of a nanotoxicity database – The MOD-ENP-TOX experience
Vriens, H;Mertens, D;Regret, R;Lin, P;Locquet, JP;Hoet, P
Abstract:The amount of experimental studies on the toxicity of nanomaterials is growing fast. Interpretation and comparison of these studies is a complex issue due to the high amount of variables possibly determining the toxicity of nanomaterials.Qualitative databases providing a structured combination, integration and quality evaluation of the existing data could reveal insights that cannot be seen from different studies alone.(more)


Usefulness of overnight pulse oximeter as the sleep assessment tool to assess the 6-year risk of road traffic collision: Evidence from the Taiwan Bus Driver Cohort Study
Wu, WT;Tsai, SS;Liao, HY;Lin, YJ;Lin, MH;Wu, TN;Shih, TS;Liou, SH
Abstract: BACKGROUND: In order to support health service organizations in arranging a system for prevention of road traffic collisions (RTC), it is important to study the usefulness of sleep assessment tools. A cohort study was used to evaluate the effectiveness of subjective and objective sleep assessment tools to assess for the 6-year risk of both first RTC event only and recurrent RTC events. METHODS: The Taiwan Bus Driver Cohort Study (TBDCS) recruited 1650 professional drivers from a large bus company in Taiwan in 2005. (more)


Does exposure to phthalates influence thyroid function and growth hormone homeostasis? The Taiwan Environmental Survey for Toxicants (TEST) 2013
Huang, HB;Pan, WH;Chang, JW;Chiang, HC;Guo, YL;Jaakkola, JJ;Huang, PC
Abstract:BACKGROUND: Previous epidemiologic and toxicological studies provide some inconsistent evidence that exposure to phthalates may affect thyroid function and growth hormone homeostasis. OBJECTIVE: To assess the relations between exposure to phthalates and indicators of thyroid function and growth hormone homeostasis disturbances both among adults and minors. METHODS: We conducted a population-based cross-sectional study of 279 Taiwanese adults (>/=18 years old) and 79 minors (<18 years old) in 2013.(more)


Characteristics, sources, and health risks of atmospheric PM2.5-bound polycyclic aromatic hydrocarbons in Hsinchu, Taiwan
Yang, TT;Hsu, CY;Chen, YC;Young, LH;Huang, CH;Ku, CH
Abstract: This study investigated PM2.5-bound polycyclic aromatic hydrocarbons (PAHs) in order to determine the seasonal changes in total benzo[a]pyrene equivalent (BaPeq) concentrations and to identify contamination sources by using a positive matrix factorization model, a conditional probability function, and characteristic ratios of PAHs in Hsinchu. The sampling period was from September 2014 to August 2015. PM2.5 samplers equipped with 47-mm quartz membrane filters were operated at a flow rate of 16.7 L min-1 for 48 h. (more)


Comparative microarray analyses of mono(2-ethylhexyl)phthalate impacts on fat cell bioenergetics and adipokine network
Chiang, HC;Wang, CH;Yeh, SC;Lin, YH;Kuo, YT;Liao, CW;Tsai, FY;Lin, WY;Chuang, WH;Tsou, TC
Abstract:Cellular accumulation of mono(2-ethylhexyl)phthalate (MEHP) has been recently demonstrated to disturb fat cell energy metabolism; however, the underlying mechanism remained unclear. The study aimed to determine how MEHP influenced fat cell transcriptome and how the changes might contribute to bioenergetics. Because of the pivotal role of PPARgamma in energy metabolism of fat cells, comparative microarray analysis of gene expression in 3T3-L1 adipocytes treated with both MEHP and rosiglitazone was performed. (more)


Depressive, anxiety and post-traumatic stress disorders at six years after occupational injuries
Chin, WS;Shiao, JSC;Liao, SC;Kuo, CY;Chen, CC;Guo, YL
Abstract: The aim of this study is to determine the prevalence rates of depressive, anxiety and PTSDs, and the risk factors for psychological symptoms at 6 years after occupational injury. This longitudinal study followed workers who were occupationally injured in 2009. Psychological symptoms and return to work were assessed at 3 and 12 months after injury. Injured workers who had completed the initial questionnaire survey at 3 or 12 months after injury were recruited. (more)


Workplace justice and intention to leave the nursing profession
Chin, W;Guo, YL;Hung, YJ;Hsieh, YT;Wang, LJ;Shiao, JS
Abstract:BACKGROUND: Poor psychosocial work environments are considered critical factors of nurses’ intention to leave their profession. Workplace injustice has been proven to increase the incidence of psychiatric morbidity among workers. However, few studies have directly investigated the effect of workplace justice on nurses’ intention to leave their profession and the population attributable risk among nurses. (more)


Aryl hydrocarbon receptor regulates histone deacetylase 8 expression to repress tumor suppressive activity in hepatocellular carcinoma
Wang, LT;Chiou, SS;Chai, CY;Hsi, E;Wang, SN;Huang, SK;Hsu, SH
Abstract: Histone deacetylase 8 (HDAC8), a unique member of class I histone deacetylases, shows remarkable correlation with advanced disease stage and multiple malignant tumors However, little is known about the contribution of HDAC8 to the tumorigenesis of hepatocellular carcinoma (HCC). The present study investigated the expression of HDAC8 regulated by the aryl hydrocarbon receptor (AHR) in HCC cell lines and tissues, and the roles of HDAC8 overexpression in cell proliferation, including potentially underlying mechanisms.(more)


An Interaction between arsenic-induced epigenetic modification and inflammatory promotion in a skin equivalent during arsenic carcinogenesis
Liao, WT;Lu, JH;Lee, CH;Lan, CE;Chang, JG;Chai, CY;Yu, HS
Abstract:Animal studies have shown that chemical carcinogenesis consists of a 3-stage process: initiation, promotion, and progression. However, due to the lack of a suitable tissue model, the molecular mechanisms of cell-cell interactions involved in those processes remain unclear. We have established a human intraepidermal carcinoma skin equivalent (SE) with organotypic culture – consisting of keratinocytes (KCs), fibroblasts, and peripheral blood mononuclear cells (PBMCs) – induced by arsenic treatment.(more)


Effect of CYP3A4 genetic polymorphisms on the genotoxicity of 4,4′-methylene-bis(2-chloroaniline)-exposed workers
Wang, CC;Chen, WL;Hsiung, CN;Chiang, ST;Wang, YC;Loh, CH;Lin, IS;Chen, HI;Liou, SH
Abstract: OBJECTIVES: We investigated the relationship between 4,4′-methylene-bis(2-chloroaniline) (MBOCA) exposure and micronucleus (MN) frequency, and how this association was affected by genetic polymorphism of the cytochrome P450 enzyme (CYP3A4). METHODS: We divided the study population into an exposed group (n=44 with total urine MBOCA >/=20 mug/g creatinine) and a control group (n=47 with total urine MBOCA <20 mug/g creatinine). (more)


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